Abstract
The activation, localization, phenotypic changes, and function of CFSE-labeled naive influenza-specific CD8 + and CD4 + T cells following influenza infection were examined. Response of adoptively transferred CD8 + T cells was seen earliest in draining lymph node. Highly activated cells were found later in the lung, airways, and spleen, were cytolytic, and expressed IFN-γ upon restimulation. Similar amounts of division at early time points, but higher numbers of CD8 + T cells, were detected at 9 and 30 days postinfection after cotransfer of CD4 + and CD8 + T cells followed by infection. Transfer of much smaller numbers of CD4 + and CD8 + T cells led to more extensive expansion but the same difference in final number between the two cell types. These studies demonstrate how CD8 + and CD4 + T cells respond to influenza at early time points postinfection and the differential kinetics of antigen-specific CD4 + and CD8 + T cells.
Original language | English (US) |
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Pages (from-to) | 89-100 |
Number of pages | 12 |
Journal | Clinical Immunology |
Volume | 113 |
Issue number | 1 |
DOIs | |
State | Published - Oct 2004 |
Externally published | Yes |
Keywords
- BAL
- Cell surface molecules
- Cellular activation
- Cellular proliferation
- DLN
- HA
- NDLN
- T lymphocytes
- Virus infection
- bronchoalveolar lavage
- draining lymph nodes
- hemagglutinin
- nondraining lymph nodes
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology