TY - JOUR
T1 - cDNA cloning and expression analysis of NeuroD mRNA in human retina
AU - Acharya, Harsha R.
AU - Dooley, Constance M.
AU - Thoreson, Wallace B.
AU - Ahmad, Iqbal
N1 - Funding Information:
We thank Anand Swaroop for the gift of human retinal cDNA libraries, the California Regional Primate Research Center at Davis for fetal monkey retina, and the Nebraska Lion's Eye Bank for human retina. This work was supported by NEI Grant R29 10313.
PY - 1997/4/17
Y1 - 1997/4/17
N2 - We have shown that bHLH proteins are involved in mammalian retinal development. Here we report the identification and analysis of the expression of a neurogenic differentiation gene, NeuroD, in human retina. In situ hybridization and immunocytochemical analyses of adult retina showed that NeuroD transcripts and NeuroD immunoreactivity are predominantly localized to the outer nuclear layer which contains the photoreceptors. Southern analysis of PCR-amplified cDNA revealed that NeuroD mRNA is also expressed in fetal human retina. Fetal monkey retina was used to analyse the spatial distribution of NeuroD in the developing retina. Both NeuroD transcripts and immunoreactivity are largely detected in the outer neuroblastic layer. Therefore, NeuroD may be involved in the differentiation as well as maintenance of the differentiated properties of photoreceptors.
AB - We have shown that bHLH proteins are involved in mammalian retinal development. Here we report the identification and analysis of the expression of a neurogenic differentiation gene, NeuroD, in human retina. In situ hybridization and immunocytochemical analyses of adult retina showed that NeuroD transcripts and NeuroD immunoreactivity are predominantly localized to the outer nuclear layer which contains the photoreceptors. Southern analysis of PCR-amplified cDNA revealed that NeuroD mRNA is also expressed in fetal human retina. Fetal monkey retina was used to analyse the spatial distribution of NeuroD in the developing retina. Both NeuroD transcripts and immunoreactivity are largely detected in the outer neuroblastic layer. Therefore, NeuroD may be involved in the differentiation as well as maintenance of the differentiated properties of photoreceptors.
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U2 - 10.1006/bbrc.1997.6483
DO - 10.1006/bbrc.1997.6483
M3 - Article
C2 - 9144558
AN - SCOPUS:0031575930
SN - 0006-291X
VL - 233
SP - 459
EP - 463
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -