Cell and tissue-specific expression of a heterologous gene under control of the myelin basic protein gene promoter in transgenic mice

Robin Miskimins, Loren Knapp, Michael J. Dewey, Xiaoping Zhang

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Myelin basic protein (MBP) is the second most abundant protein in CNS myelin. We have used transgenic mice to investigate the ability of the 5′ flanking sequence of the mouse MBP gene to regulate the cell-type-specific- and temporal expression of a heterologous gene under its control. Transgenic mice were produced with a construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene downstream of the MBP 5′ flanking sequence and CAT expression was monitored both enzymatically and histochemically. The results indicate that 1323 bp of 5′ flanking is sufficient to direct CAT expression specifically to the tissue and cell-type, in which MBP is normally synthesized. Additionally, this length of the sequence also retains the ability to temporally regulate CAT levels in a manner analogous to endogenous MBP levels.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalDevelopmental Brain Research
Volume65
Issue number2
DOIs
StatePublished - Feb 21 1992

Keywords

  • CAT gene
  • Myelin basic protein
  • Oligodendrocyte-specific expression
  • Transgenic mice

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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