Cell-based bio-optical sensors using chalcogenide fibers

Pierre Lucas, Allison A. Wilhelm, Mark R. Riley, Diana L. Derosa, Jayne M. Collier

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations


An optical bio-sensor is built based on an infrared chalcogenide fiber coated with live cells. The fiber is immersed in an aqueous media appropriate for cell viability. The response of the cells to small quantities of toxicant can be monitored spectroscopically. The properties of chalcogenide fibers used for cell-based biosensors are investigated. The chemical stability of Te-As-Se fibers in aqueous media is shown to depend on the previous storage time of the fiber. Older fibers are shown to generate an oxide layer during extended exposure to air. This layer readily dissolves in aqueous solution and causes the release of As in the cell environment. The release of As during dissolution of the oxide layer is measured with ICP-MS and is shown to be complete after a couple hours. Fresh fibers do not show any detectable oxide layer and show excellent stability in aqueous solution. The surface roughness of old and fresh fibers is investigated with AFM before and after dissolution in aqueous media. Old fibers immersed in solution show sizable roughness due to the oxide surface layer dissolution. Fresh fibers do not show any detectable changes even after extended immersion in aqueous solution. The toxicity of As to various types of vertebrate cells is quantified using a colorimetric assay. Old fibers are shown to be notably toxic due to As released during dissolution. The fiber toxicity is shown to decrease when the fibers are previously washed in solution. The toxicity of the resulting wash water is then shown to increase due to the increase in As concentration.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2006
Externally publishedYes
EventOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications VI - San Jose, CA, United States
Duration: Jan 21 2006Jan 22 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications VI
Country/TerritoryUnited States
CitySan Jose, CA


  • Arsenic toxicity
  • Bio-compatibility
  • Bio-sensor
  • Chalcogenide glass fiber
  • Corrosion
  • Evanescent wave
  • Human lung cell
  • IR spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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