Cell-cell contacts with epithelial cells modulate the phenotype of human macrophages

I. Stříž, A. Slavčev, J. Kalanin, M. Jarešová, S. I. Rennard

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Interactions of macrophages with epithelium represent one of the pathways involved in regulating local immune mechanisms. We studied the effect of cell-cell contact with an epithelial monolayer on the phenotype of macrophages. Human monocytes and THP-1 macrophages were co-cultured with monolayers of human bronchial epithelial cells (HBECs), the alveolar type II-like cell line A549, renal adenocarcinoma epithelial cells (RA), and the lung fibroblast strain HFL-1. The expression of CD11b, CD14, CD54, and HLA-DR was measured by immunocytochemistry and flow cytometry and showed epithelial cell induction of CD54 and HLA-DR in monocytes and of all antigens in THP-1 cells. Co-culture with fibroblasts did not change the phenotype of macrophages. Separation by a filter insert inhibited most of the effects. Culture supernatants did not induce prominent phenotypic changes. Cell-cell contacts with epithelium appear to be of importance in regulating the phenotype of macrophages.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
Issue number4
StatePublished - 2001


  • Epithelial cells
  • Macrophages
  • Phenotype

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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