Cell-directed assembly of lipid-silica nanostructures providing extended cell viability

Helen K. Baca, Carlee Ashley, Eric Carnes, Deanna Lopez, Jeb Hemming, Darren Dunphy, Seema Singh, Zhu Chen, Nanguo Liu, Hongyou Fan, Gabriel P. López, Susan M. Brozik, Margaret Werner-Washburne, C. Jeffrey Brinker

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

Amphiphilic phospholipids were used to direct the formation of biocompatible, uniform silica nanostructures in the presence of Saccharomyces cerevisiae and bacterial cell lines. The cell surfaces organize multilayered phospholipid vesicles that interface coherently with the silica host and help relieve drying stresses that develop with conventional templates. These host structures maintain cell accessibility, addressability, and viability in the absence of buffer or an external fluidic architecture. The cell surfaces are accessible and can be used to localize added proteins, plasmids, and nanocrystals. Prolonged cell viability combined with reporter protein expression enabled stand-alone cell-based sensing.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalScience
Volume313
Issue number5785
DOIs
StatePublished - Jul 21 2006
Externally publishedYes

ASJC Scopus subject areas

  • General

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