Cell-directed integration into three-dimensional Lipid-Silica nanostructured matrices

Jason C. Harper, Constantine Y. Khirpin, Eric C. Carnes, Carlee E. Ashley, Deanna M. Lopez, Travis Savage, Howland D.T. Jones, Ryan W. Davis, Dominique E. Nunez, Lina M. Brinker, Bryan Kaehr, Susan M. Brozik, C. Jeffrey Brinker

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We report a unique approach in which living cells direct their integration into 3D solid-state nanostructures. Yeast cells deposited on a weakly condensed lipid/silica thin film mesophase actively reconstruct the surface to create a fully 3D bio/nano interface, composed of localized lipid bilayers enveloped by a lipid/silica mesophase, through a self-catalyzed silica condensation process. Remarkably, this integration process selects exclusively for living cells over the corresponding apoptotic cells (those undergoing programmed cell death), via the development of a pH gradient, which catalyzes silica deposition and the formation of a coherent interface between the cell and surrounding silica matrix. Added long-chain lipids or auxiliary nanocomponents are localized within the pH gradient, allowing the development of complex active and accessible bio/nano interfaces not achievable by other synthetic methods. Overall, this approach provides the first demonstration of active cell-directed integration into a nominally solid-state three-dimensional architecture. It promises a new means to integrate "bio" with "nano" into platforms useful to study and manipulate cellular behavior at the individual cell level and to interface living organisms with electronics, photonics, and fluidics.

Original languageEnglish (US)
Pages (from-to)5539-5550
Number of pages12
JournalACS Nano
Volume4
Issue number10
DOIs
StatePublished - Oct 26 2010
Externally publishedYes

Keywords

  • biomaterials
  • cell encapsulation/entrapment
  • cell-directed integration
  • evaporation-induced self-assembly
  • mesoporous silica
  • sol-gel

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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