Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes

Nicole A. Haverland, Matthew Waas, Ioanna Ntai, Theodore Keppel, Rebekah L. Gundry, Neil L. Kelleher

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Lymphocytes are immune cells that are critical for the maintenance of adaptive immunity. Differentiation of lymphoid progenitors yields B-, T-, and NK-cell subtypes that individually correlate with specific forms of leukemia or lymphoma. Therefore, it is imperative a precise method of cell categorization is utilized to detect differences in distinct disease states present in patients. One viable means of classification involves evaluation of the cell surface proteome of lymphoid malignancies. Specifically, this manuscript details the use of an antibody independent approach known as Cell Surface Capture Technology, to assess the N-glycoproteome of four human lymphocyte cell lines. Altogether, 404 cell surface N-glycoproteins were identified as markers for specific cell types involved in lymphocytic malignancies, including 82 N-glycoproteins that had not been previously been described for B or T cells within the Cell Surface Protein Atlas. Comparative analysis, hierarchical clustering techniques, and label-free quantitation were used to reveal proteins most informative for each cell type. Undoubtedly, the characterization of the cell surface proteome of lymphoid malignancies is a first step toward improving personalized diagnosis and treatment of leukemia and lymphoma.

Original languageEnglish (US)
Article number1700156
JournalProteomics
Volume17
Issue number19
DOIs
StatePublished - Oct 2017

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Keywords

  • N-glycoproteins
  • cell surface proteins
  • plasma membrane

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Haverland, N. A., Waas, M., Ntai, I., Keppel, T., Gundry, R. L., & Kelleher, N. L. (2017). Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes. Proteomics, 17(19), [1700156]. https://doi.org/10.1002/pmic.201700156