TY - JOUR
T1 - Cellular distribution of lens epithelium-derived growth factor (LEDGF) in the rat eye
T2 - Loss of LEDGF from nuclei of differentiating cells
AU - Kubo, Eri
AU - Singh, Dhirendra P.
AU - Fatma, Nigar
AU - Shinohara, Toshimichi
AU - Zelenka, Peggy
AU - Reddy, Venkat N.
AU - Chylack, Leo T.
N1 - Funding Information:
Acknowledgements The work was supported by NIH grants EY12015, EY10824, EY13394, and EY07003, and the Massachusetts Lions Eye Research Fund.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Lens epithelium-derived growth factor (LEDGF) enhances the survival and growth of cells. To understand LEDGF's spatial localization and its putative function(s) during proliferation and differentiation, we localized LEDGF during terminal differentiation in whole rat lenses, lens epithelial cell (LEC) explants stimulated with FGF-2, and insulin, iris, human LECs with lentoids. In addition, intracellular localization of LEDGF was performed in other ocular tissues: ciliary body, retina, and cornea. We found the immunopositivity of nuclear LEDGF decreased in LECs of the equatorial region. In contrast, immunopositivity of LEDGF was detected in the cytoplasm of LECs and superficial fiber cells. After treating LEC explants with FGF-2 and insulin, which are known to be differentiating factors for LECs, the nuclei of these cells showed no LEDGF immunopositivity, but explants did express p57kip2, a differentiation marker protein. Also, immunopositive LEDGF was not detected in the nuclei of differentiated cells, lentoid body, and corneal epithelial cells. This demonstrated that the loss of LEDGF from the nucleus may be associated with the process of terminal differentiation that might be in some way common with the biochemical mechanisms of apoptosis. The spatial and temporal distribution of LEDGF in the present study also provides a vision for further investigation as to how this protein is involved in cell fate determination.
AB - Lens epithelium-derived growth factor (LEDGF) enhances the survival and growth of cells. To understand LEDGF's spatial localization and its putative function(s) during proliferation and differentiation, we localized LEDGF during terminal differentiation in whole rat lenses, lens epithelial cell (LEC) explants stimulated with FGF-2, and insulin, iris, human LECs with lentoids. In addition, intracellular localization of LEDGF was performed in other ocular tissues: ciliary body, retina, and cornea. We found the immunopositivity of nuclear LEDGF decreased in LECs of the equatorial region. In contrast, immunopositivity of LEDGF was detected in the cytoplasm of LECs and superficial fiber cells. After treating LEC explants with FGF-2 and insulin, which are known to be differentiating factors for LECs, the nuclei of these cells showed no LEDGF immunopositivity, but explants did express p57kip2, a differentiation marker protein. Also, immunopositive LEDGF was not detected in the nuclei of differentiated cells, lentoid body, and corneal epithelial cells. This demonstrated that the loss of LEDGF from the nucleus may be associated with the process of terminal differentiation that might be in some way common with the biochemical mechanisms of apoptosis. The spatial and temporal distribution of LEDGF in the present study also provides a vision for further investigation as to how this protein is involved in cell fate determination.
KW - Differentiation
KW - Immunohistochemistry
KW - In situ hybridization
KW - LEC explants
KW - LEDGF
KW - Lentoid body
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U2 - 10.1007/s00418-003-0518-3
DO - 10.1007/s00418-003-0518-3
M3 - Article
C2 - 12692670
AN - SCOPUS:0037688178
VL - 119
SP - 289
EP - 299
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 4
ER -