Cellular fibronectin stimulates hepatocytes to produce factors that promote alcohol-induced liver injury

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Aim: To examine the consequences of cellular fibronectin (cFn) accumulation during alcohol-induced injury, and investigate whether increased cFn could have an effect on hepatocytes (HCs) by producing factors that could contribute to alcohol-induced liver injury. Methods: HCs were isolated from rats fed a control or ethanol liquid diet for four to six weeks. Exogenous cFn (up to 7.5 μg/mL) was added to cells cultured for 20 h, and viability (lactate dehydrogenase), apoptosis (caspase activity) and secretion of proinflammatory cytokines (tumor necrosis factor alpha, TNF-a and interleukin 6, IL-6), matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitors of metalloproteinases, TIMPs) was determined. Degradation of iodinated cFn was determined over a 3 h time period in the preparations. Results: cFn degradation is impaired in HCs isolated from ethanol-fed animals, leading to its accumulation in the matrix. Addition of exogenous cFn did not affect viability of HCs from control or ethanol-fed animals, and apoptosis was affected only at the higher concentration. Secretion of MMPs, TIMPs, TNF-a and IL-6, however, was increased by exogenously added cFn, with HCs from ethanol-fed animals showing increased susceptibility compared to the controls. Conclusion: These results suggest that the elevated amounts of cFn observed in alcoholic liver injury can stimulate hepatocytes to produce factors which promote further tissue damage.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalWorld Journal of Hepatology
Volume3
Issue number2
DOIs
StatePublished - 2011

Keywords

  • Alcoholic liver diseases
  • Asialoglycoprotein receptor
  • Fibronectin
  • Fibrosis
  • Hepatocytes
  • Inflammation

ASJC Scopus subject areas

  • Hepatology

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