Cellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes

Ruiguo Yang, Bo Song, Zhiyong Sun, King Wai Chiu Lai, Carmen Kar Man Fung, Kevin C. Patterson, Kristina Seiffert-Sinha, Animesh A. Sinha, Ning Xi

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • Atomic Force Microscopy
  • Cell-cell adhesion
  • Desmosome
  • Intermediate filament
  • Mechanical property
  • Nanosurgery

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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  • Cite this

    Yang, R., Song, B., Sun, Z., Lai, K. W. C., Fung, C. K. M., Patterson, K. C., Seiffert-Sinha, K., Sinha, A. A., & Xi, N. (2015). Cellular level robotic surgery: Nanodissection of intermediate filaments in live keratinocytes. Nanomedicine: Nanotechnology, Biology, and Medicine, 11(1), 137-145. https://doi.org/10.1016/j.nano.2014.08.008