Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment

Wojciech Rozek, Mary Ricardo-Dukelow, Sondra Holloway, Howard E. Gendelman, Valerie Wojna, Loyda M. Melendez, Pawel Ciborowski

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Advanced HIV-1 infection is commonly associated with progressive immune suppression and the development of cognitive, motor, and behavior disturbances. In its most severe form, it is diagnosed as HIV-1 associated dementia (HAD) and can progress to profound functional disability and death. Despite prodigious efforts to uncover biomarkers of HAD, none can adequately reflect disease onset or progression. Thus, we developed a proteomics platform for HAD biomarker discovery and used it to perform a pilot study on cerebrospinal fluid (CSF) from HIV-1-infected people with or without HAD. A 2-dimensional electrophoresis (2-DE) map of a HAD CSF proteome was focused on differentially expressed proteins. 2-DE difference gel electrophoresis (2-D DIGE) analysis showed >90 differences in protein spots of which 20 proteins were identified. Differential expression of 6 proteins was validated by Western blot tests and included vitamin D binding protein, clusterin, gelsolin, complement C3, procollagen C-endopeptidase enhancer 1, and cystatin C. We posit that these proteins, alone or together, are potential HAD biomarkers.

Original languageEnglish (US)
Pages (from-to)4189-4199
Number of pages11
JournalJournal of proteome research
Volume6
Issue number11
DOIs
StatePublished - Nov 1 2007

Keywords

  • 2-dimensional electrophoresis
  • Cerebrospinal fluid
  • Cognitive impairment
  • DIGE
  • HAD
  • HIV
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Cerebrospinal fluid proteomic profiling of HIV-1-infected patients with cognitive impairment'. Together they form a unique fingerprint.

  • Cite this