TY - JOUR
T1 - Cerulenin inhibition of spore germination in Rhizopus stolonifer
AU - Nickerson, Kenneth W.
AU - Leastman, Elaine
N1 - Funding Information:
the spores used and for helpful discussions. This research was assisted by funds from NIH Biochemical Research Support Grant RR-07055 and by Public Health Service Grant AI-108057 to Dr. James Van Etten.
PY - 1978/3
Y1 - 1978/3
N2 - We have used cerulenin, a known inhibitor of fatty acid synthesis, to study essential fatty acid and membrane syntheses during spore germination. Cerulenin (4 μg/ml) prevented the germination of Rhizopus stolonifer sporangiospores and this inhibition was reversed by Tween 80. Specifically, spore swelling, germ tube formation, and, probably, mitochondrial biogenesis were inhibited, whereas, during the first 2 h at least, the rates of protein and RNA syntheses and oxygen uptake remained undiminished. We conclude that de novo fatty acid synthesis is necessary for spore germination.
AB - We have used cerulenin, a known inhibitor of fatty acid synthesis, to study essential fatty acid and membrane syntheses during spore germination. Cerulenin (4 μg/ml) prevented the germination of Rhizopus stolonifer sporangiospores and this inhibition was reversed by Tween 80. Specifically, spore swelling, germ tube formation, and, probably, mitochondrial biogenesis were inhibited, whereas, during the first 2 h at least, the rates of protein and RNA syntheses and oxygen uptake remained undiminished. We conclude that de novo fatty acid synthesis is necessary for spore germination.
KW - Rhizopus stolonifer
KW - cerulenin
KW - glyoxalate cycle
KW - membrane synthesis
KW - mitochondrial biogenesis
KW - spore germination
UR - http://www.scopus.com/inward/record.url?scp=0343372407&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0343372407&partnerID=8YFLogxK
U2 - 10.1016/S0147-5975(78)80038-3
DO - 10.1016/S0147-5975(78)80038-3
M3 - Article
AN - SCOPUS:0343372407
VL - 2
SP - 26
EP - 31
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 1
ER -