Changes in messenger RNAs and protein synthesis during germination of Rhizopus stolonifer sporangiospores

Shelby N. Freer, James L. Van Etten

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Sporangiospores of Rhizopus stolonifer were pulse-labeled with [35S]methionine at various times during germination in order to identify germination-specific proteins. In addition, the in vivo synthesized proteins were compared with in vitro synthesized products encoded by either total messenger RNA or polysomal messenger RNA isolated at comparable time periods to determine if the majority of the proteins synthesized during spore germination were under transcriptional or translational control. We conclude that, with few exceptions, the majority of the proteins synthesized were under transcriptional control. A few proteins were synthesized only at discrete time periods during the germination process and, thus, may be germination-specific proteins. However, at no time during germination did we find a germination-specific protein synthesized which represented a major fraction of the total proteins synthesized.

Original languageEnglish (US)
Pages (from-to)313-325
Number of pages13
JournalExperimental Mycology
Volume2
Issue number4
DOIs
Publication statusPublished - Dec 1978

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Keywords

  • Messenger RNA
  • Rhizopus stolonifer
  • proteins
  • spores
  • transcriptional control
  • translational control

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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