Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores

Cheng Shung Gong; James L. Van Etten

doi:10.1016/0005-2787(72)90031-7

Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores

Cheng Shung Gong, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 2.7.7.6) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.

Original language	English (US)
Pages (from-to)	44-52
Number of pages	9
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	272
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(72)90031-7
State	Published - Jun 22 1972

ASJC Scopus subject areas

Medicine(all)

Access to Document

10.1016/0005-2787(72)90031-7

Cite this

@article{f5c20aa9522a45328c4ed947487cc7e3,

title = "Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores",

abstract = "Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 2.7.7.6) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.",

author = "Gong, {Cheng Shung} and {Van Etten}, {James L.}",

note = "Funding Information: The authors are indebted to the technical assistance of H. R. Roker and W. J. Haeffner. This investigation was supported by Public Health Service grant Aio8o57 from the National Institute of Allergy and Infectious Diseases.",

year = "1972",

month = jun,

day = "22",

doi = "10.1016/0005-2787(72)90031-7",

language = "English (US)",

volume = "272",

pages = "44--52",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

issn = "0005-2787",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores

AU - Gong, Cheng Shung

AU - Van Etten, James L.

N1 - Funding Information: The authors are indebted to the technical assistance of H. R. Roker and W. J. Haeffner. This investigation was supported by Public Health Service grant Aio8o57 from the National Institute of Allergy and Infectious Diseases.

PY - 1972/6/22

Y1 - 1972/6/22

N2 - Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 2.7.7.6) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.

AB - Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 2.7.7.6) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.

UR - http://www.scopus.com/inward/record.url?scp=0015519782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015519782&partnerID=8YFLogxK

U2 - 10.1016/0005-2787(72)90031-7

DO - 10.1016/0005-2787(72)90031-7

M3 - Article

C2 - 5043713

AN - SCOPUS:0015519782

SN - 0005-2787

VL - 272

SP - 44

EP - 52

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 1

ER -

Changes in soluble ribonucleic acid polymerases associated with the germination of Rhizopus stolonifer spores

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this