Germinated spores of Rhizopus stolonifer contained three soluble DNA-directed RNA polymerase enzymes (nucleoside triphosphate : RNA nucleotidyltransferase, EC 188.8.131.52) which were similar in many of their characteristics to those obtained from other eukaryotic organisms. Preparations from ungerminated spores yielded only RNA polymerases I and III. RNA polymerase III from both spore states exhibited identical characteristics, whereas RNA polymerase I from the ungerminated spores responded to divalent cations differently than the corresponding fraction from germinated spores. RNA polymerase II was first observed after the spores had undergone a swelling phase and they were ready to initiate germ tube formation. At the same time the characteristics of RNA polymerase I were altered so that they resembled those of the germinated spore enzyme.
|Original language||English (US)|
|Number of pages||9|
|Journal||BBA Section Nucleic Acids And Protein Synthesis|
|State||Published - Jun 22 1972|
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