TY - JOUR
T1 - Changes in the messenger RNA population from the heart of cardiomyopathic hamsters
AU - Ouellette, Michel
AU - Jolicoeur, Christine
AU - Brakier-Gingras, Léa
N1 - Funding Information:
We thank Drs Normand Brisson, Gabriel Gingras and Michael Laughrea for helpful discussion and comments. We are grateful to Drs G. Jasmin and H. Stephens for their interest in this work. This study was supported by the Canadian Heart Foundation and the Banting Research Foundation. M. Ouellette and C. Jolicoeur are the recipients of a studentship from the Medical Research Council of Canada and the Fonds de la Recherche en Sant~ du Quebec, respectively.
PY - 1987/7
Y1 - 1987/7
N2 - Messenger RNAs were extracted from the heart of cardiomyopathic hamsters at different phases of the disease and from age-matched control hamsters. They were translated into proteins in a rabbit reticulocyte lysate in the presence of [35S]methionine, the translational products were fractionated by two-dimensional polyacrylamide gel electrophoresis and analyzed by fluorography. No difference between cardiomyopathic and control hamsters could be detected when comparing the spots corresponding to the major contractile proteins. However, we observed that three translational products of minor abundance were reproducibly decreased in cardiomyopathic hamsters at 60 days (necrotic phase) and 200 days (final phase) but not at 30 days (prenecrotic phase). At 120 days (hypertrophic phase), the decrease could also be detected but was much less pronounced.
AB - Messenger RNAs were extracted from the heart of cardiomyopathic hamsters at different phases of the disease and from age-matched control hamsters. They were translated into proteins in a rabbit reticulocyte lysate in the presence of [35S]methionine, the translational products were fractionated by two-dimensional polyacrylamide gel electrophoresis and analyzed by fluorography. No difference between cardiomyopathic and control hamsters could be detected when comparing the spots corresponding to the major contractile proteins. However, we observed that three translational products of minor abundance were reproducibly decreased in cardiomyopathic hamsters at 60 days (necrotic phase) and 200 days (final phase) but not at 30 days (prenecrotic phase). At 120 days (hypertrophic phase), the decrease could also be detected but was much less pronounced.
KW - Cardiac hypertrophy and heart failure
KW - Cardiomyopathy
KW - Messenger RNA
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U2 - 10.1016/S0022-2828(87)80379-6
DO - 10.1016/S0022-2828(87)80379-6
M3 - Article
C2 - 3682008
AN - SCOPUS:0023275342
SN - 0022-2828
VL - 19
SP - 709
EP - 717
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 7
ER -