Changes of calcium channel mRNA, protein and current in NG108-15 cells after cell differentiation

Jinxu Liu, Huiyin Tu, Dongze Zhang, Yu Long Li

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Based on the characteristics of differentiated NG108-15 cells (cell membrane excitability, acetylcholine release, and activities of choline acetyltransferase and acetylcholinesterase), NG108-15 cells are extensively used to explore neuronal functions as a cholinergic cell line. In the present study, differentiation-induced alterations of voltage-gated Ca2+ channel mRNA, protein, and current were investigated in the NG108-15 cells. Real-time PCR, Western blot, and whole-cell patch-clamp data showed that differentiation caused mRNA, protein, and ion current changes of all Ca2+ channel subunits. However, the changes of mRNA, protein, and ion current are inconsistent in all Ca2+ channel subunits. Especially, P/Q- and R-type Ca2+ channel proteins do not form the functional P/Q- and R-type Ca2+ channels even if the mRNA and protein of P/Q- and R-type Ca2+ channels can be detected in NG108-15 cells. These results indicate that differentiation can modulate gene transcription, protein translation, and post-translation of the Ca2+ channels to induce the alteration of the Ca2+ ion currents in NG108-15 cells. From these data, we understand that combining real-time PCR, Western blot, and patch-clamp techniques can comprehensively unveil the modulation of the Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume423
Issue number1
DOIs
StatePublished - Jun 22 2012

Keywords

  • Acetylcholine
  • Ca channel
  • NG108-15 cell
  • Patch clamp
  • Real-time RT-PCR
  • Western blot

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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