@article{6b3bd15dda194dfea11476da1c028bec,
title = "Characterization and small RNA content of extracellular vesicles in follicular fluid of developing bovine antral follicles",
abstract = "Exosomes and microvesicles (i.e., extracellular vesicles: EVs) have been identified within ovarian follicular fluid and recent evidence suggests that EVs are able to elicit profound effects on ovarian cell function. While existence of miRNA within EVs has been reported, whether EV size and concentration as well as their cargos (i.e., proteins and RNA) change during antral follicle growth remains unknown. Extracellular vesicles isolated from follicular fluid of small, medium and large bovine follicles were similar in size, while concentration of EVs decreased progressively as follicle size increased. Electron microscopy indicated a highly purified population of the lipid bilayer enclosed vesicles that were enriched in exosome biomarkers including CD81 and Alix. Small RNA sequencing identified a large number of known and novel miRNAs that changed in the EVs of different size follicles. Ingenuity Pathway Analysis (IPA) indicated that miRNA abundant in small follicle EV preparations were associated with cell proliferation pathways, while those miRNA abundant in large follicle preparations were related to inflammatory response pathways. These studies are the first to demonstrate that EVs change in their levels and makeup during antral follicle development and point to the potential for a unique vesicle-mediated cell-to-cell communication network within the ovarian follicle.",
author = "Raphatphorn Navakanitworakul and Hung, {Wei Ting} and Sumedha Gunewardena and Davis, {John S.} and Wilaiwan Chotigeat and Christenson, {Lane K.}",
note = "Funding Information: We also wish to acknowledge several Core facilities that were essential for completion of this work: University of Kansas Medical Center Electron Microscopy Research Lab facility is supported in part, by NIH COBRE grant 9P20GM104936. The JEOL JEM-1400 TEM used in the study was purchased with funds from NIH grant S10RR027564; University of Kansas Medical CenterGenomics Core for generating the sequencing data sets. The Genomics Core is supported by the University of Kansas Medical CenterSchool of Medicine and the Kansas Intellectual and Developmental Disability Research Center (HD02528); and the University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core which is supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (P50-HD28934). Funding: National Institute of Child Health and Human Development: HD061580 and HD082484 to LKC and the University of Kansas Medical Center Genomics Core which is supported by Kansas Intellectual and Developmental Disability Research Center (National Institute of Child Health and Human Development-HD02528) and COBRE P20 RR024214. This work was also supported in part by the Olson Center for Womens Health and the United States (U.S.) Department of Veterans Affairs Biomedical Laboratory Research and Development Service (JSD).",
year = "2016",
month = may,
day = "9",
doi = "10.1038/srep25486",
language = "English (US)",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}