Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family

Kathleen M. Fisch, Jessica L. Petersen, Melinda R. Baerwald, John K. Pedroia, Bernie May

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy-Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy-Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt (Spirinchus thaleichthys).

Original languageEnglish (US)
Pages (from-to)405-408
Number of pages4
JournalMolecular Ecology Resources
Volume9
Issue number1
DOIs
StatePublished - Jan 2009

Keywords

  • Cross-species amplification
  • Delta smelt
  • Hypomesus transpacificus
  • Microsatellites
  • Osmeridae
  • Primers

ASJC Scopus subject areas

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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