Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli

Shailesh K. Lal, Scott Johnson, Tyrrell Conway, Philip M. Kelley

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

A cDNA encoding maize enolase (2-phospho-D-glycerate hydrolase) was purified by functional genetic complementation using an enolase deficient mutant of Escherichia coli, DF261. This cDNA, pZM245, was characterized by restriction mapping and DNA sequence analysis. The cDNA contained an open reading frame encoding a protein of 446 amino acids with a high degree of similarity to enolase sequences from other organisms (72% identity to yeast enolase and 82% identity to human enolase). The pZM245 contains a correctly positioned consensus prokaryotic translation initiation sequence. The specific activity of enolase in maize increases to about twice its initial level after 48 hours of anaerobiosis. Northern-blot analysis showed a five-fold anaerobic induction in enolase mRNA, while heat shock or cold shock increased enolase mRNA levels only slightly. Southern-blot analysis of maize genomic DNA indicated that there is one copy of the pZM245 hybridizing sequence per haploid genome in maize.

Original languageEnglish (US)
Pages (from-to)787-795
Number of pages9
JournalPlant Molecular Biology
Volume16
Issue number5
DOIs
StatePublished - May 1991

Keywords

  • anaerobic stress
  • enolase
  • gene regulation
  • maize

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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