Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains

Cristian F. Quispe; Ahmed Esmael; Olivia Sonderman; Michelle McQuinn; Irina Agarkova; Mohammed Battah; Garry A. Duncan; David D. Dunigan; Timothy P.L. Smith; Cristina De Castro; Immacolata Speciale; Fangrui Ma; James L. Van Etten

doi:10.1016/j.virol.2016.10.013

Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains

Cristian F. Quispe, Ahmed Esmael, Olivia Sonderman, Michelle McQuinn, Irina Agarkova, Mohammed Battah, Garry A. Duncan, David D. Dunigan, Timothy P.L. Smith, Cristina De Castro, Immacolata Speciale, Fangrui Ma, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2–3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2–3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2–3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical 2612 base-paired inverted sequence after which the DNA sequence diverged. OSy-NE5 encoded 357 predicted CDSs and 13 tRNAs. Interestingly, OSy-NE5 attached to and initiated infection in NC64A cells but infectious progeny viruses were not produced; thus OSy-NE5 replication in NC64A is blocked at some later stage of replication.

Original language	English (US)
Pages (from-to)	103-113
Number of pages	11
Journal	Virology
Volume	500
DOIs	https://doi.org/10.1016/j.virol.2016.10.013
State	Published - Jan 1 2017

Keywords

Algal viruses
Chlorella variabilis
Chloroviruses
Non-permissive cells
OSy-NE5
Permissive cells

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2016.10.013

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Quispe, C. F., Esmael, A., Sonderman, O., McQuinn, M., Agarkova, I., Battah, M., Duncan, G. A., Dunigan, D. D., Smith, T. P. L., De Castro, C., Speciale, I., Ma, F., & Van Etten, J. L. (2017). Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains. Virology, 500, 103-113. https://doi.org/10.1016/j.virol.2016.10.013

Quispe, CF, Esmael, A, Sonderman, O, McQuinn, M, Agarkova, I, Battah, M, Duncan, GA, Dunigan, DD, Smith, TPL, De Castro, C, Speciale, I, Ma, F & Van Etten, JL 2017, 'Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains', Virology, vol. 500, pp. 103-113. https://doi.org/10.1016/j.virol.2016.10.013

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title = "Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains",

abstract = "A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2–3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2–3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2–3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical 2612 base-paired inverted sequence after which the DNA sequence diverged. OSy-NE5 encoded 357 predicted CDSs and 13 tRNAs. Interestingly, OSy-NE5 attached to and initiated infection in NC64A cells but infectious progeny viruses were not produced; thus OSy-NE5 replication in NC64A is blocked at some later stage of replication.",

keywords = "Algal viruses, Chlorella variabilis, Chloroviruses, Non-permissive cells, OSy-NE5, Permissive cells",

author = "Quispe, {Cristian F.} and Ahmed Esmael and Olivia Sonderman and Michelle McQuinn and Irina Agarkova and Mohammed Battah and Duncan, {Garry A.} and Dunigan, {David D.} and Smith, {Timothy P.L.} and {De Castro}, Cristina and Immacolata Speciale and Fangrui Ma and {Van Etten}, {James L.}",

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doi = "10.1016/j.virol.2016.10.013",

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AU - Quispe, Cristian F.

AU - Esmael, Ahmed

AU - Sonderman, Olivia

AU - McQuinn, Michelle

AU - Agarkova, Irina

AU - Battah, Mohammed

AU - Duncan, Garry A.

AU - Dunigan, David D.

AU - Smith, Timothy P.L.

AU - De Castro, Cristina

AU - Speciale, Immacolata

AU - Ma, Fangrui

AU - Van Etten, James L.

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N2 - A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2–3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2–3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2–3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical 2612 base-paired inverted sequence after which the DNA sequence diverged. OSy-NE5 encoded 357 predicted CDSs and 13 tRNAs. Interestingly, OSy-NE5 attached to and initiated infection in NC64A cells but infectious progeny viruses were not produced; thus OSy-NE5 replication in NC64A is blocked at some later stage of replication.

AB - A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2–3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2–3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2–3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical 2612 base-paired inverted sequence after which the DNA sequence diverged. OSy-NE5 encoded 357 predicted CDSs and 13 tRNAs. Interestingly, OSy-NE5 attached to and initiated infection in NC64A cells but infectious progeny viruses were not produced; thus OSy-NE5 replication in NC64A is blocked at some later stage of replication.

KW - Algal viruses

KW - Chlorella variabilis

KW - Chloroviruses

KW - Non-permissive cells

KW - OSy-NE5

KW - Permissive cells

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Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains

Abstract

Keywords

ASJC Scopus subject areas

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