@article{24d2393a779e4cfd9c5850ac1fe7a6c6,
title = "Characterization of a new chlorovirus type with permissive and non-permissive features on phylogenetically related algal strains",
abstract = "A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2–3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2–3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2–3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical 2612 base-paired inverted sequence after which the DNA sequence diverged. OSy-NE5 encoded 357 predicted CDSs and 13 tRNAs. Interestingly, OSy-NE5 attached to and initiated infection in NC64A cells but infectious progeny viruses were not produced; thus OSy-NE5 replication in NC64A is blocked at some later stage of replication.",
keywords = "Algal viruses, Chlorella variabilis, Chloroviruses, Non-permissive cells, OSy-NE5, Permissive cells",
author = "Quispe, {Cristian F.} and Ahmed Esmael and Olivia Sonderman and Michelle McQuinn and Irina Agarkova and Mohammed Battah and Duncan, {Garry A.} and Dunigan, {David D.} and Smith, {Timothy P.L.} and {De Castro}, Cristina and Immacolata Speciale and Fangrui Ma and {Van Etten}, {James L.}",
note = "Funding Information: Funding for this study was partially provided by the NSF-EPSCoR Grant EPS-1004094 (JVE), the COBRE program of the National Center for Research Resources Grant P20-RR15535 (JVE), the National Center for Research Resources grant 5P20RR016469 (GAD), and the National Institute for General Medical Science grant 8P20GM103427 (GAD). OS was supported by UNL UCARE and ARD scholarships. AE was supported by the Egyptian Ministry of Higher Education (Cultural Affairs and Missions Sector). We thank Danielle Shae and Han Chen in the Morrison Core Research Facility at UNL and William Thompson and Kristen Kuhn at USDA/ARS, for technical assistance. TPLS was supported by USDA/ARS appropriated project 5438-31320-012-00D . The USDA is an equal opportunity employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. ",
year = "2017",
month = jan,
day = "1",
doi = "10.1016/j.virol.2016.10.013",
language = "English (US)",
volume = "500",
pages = "103--113",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
}