Characterization of a segmented double-helical RNA from bacteriophage φ6

J. S. Semancik, A. K. Vidaver, J. L. Van Etten

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

The nucleic acid component of bacteriophage φ6 is characterized as a double stranded RNA molecule with a buoyant density of 1.605 g/cm3 and nucleotide composition of C, 27.3%; A, 21.8%; G, 28.9%; and U, 22.0%. The hyperchromicity profile in 0.1 × SSC (SSC is 0.15 m-NaCl, 0.015 m-sodium citrate) demonstrated a rapid increase with a Tm value of 91 °C. The nucleic acid was resistant to degradation by DNase, spleen phosphodiesterase and pancreatic RNase in 2 × SSC buffer but sensitive to degradation by venom phosphodiesterase, pancreatic RNase in 0.01 × SSC and hydrolysis in KOH. Three distinct double stranded RNA species of 2.2, 2.8 and 4.5 × 106 daltons were observed after rate zonal centrifugation, polyacrylamide gel electrophoresis and electron microscopy. This communication therefore presents data establishing a new class of double stranded RNA bacteriophage.

Original languageEnglish (US)
Pages (from-to)617-622,IN19,623-625
JournalJournal of Molecular Biology
Volume78
Issue number4
DOIs
StatePublished - Aug 25 1973

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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