Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line

P. M. Kimball; M. G. Brattain; W. E. White

doi:10.1042/bj1930109

Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line

P. M. Kimball, M. G. Brattain, W. E. White

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line. The separation and characterization of the isoenzymes of N-acetyl-β-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.

Original language	English (US)
Pages (from-to)	109-113
Number of pages	5
Journal	Biochemical Journal
Volume	193
Issue number	1
DOIs	https://doi.org/10.1042/bj1930109
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1042/bj1930109

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abstract = "A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line. The separation and characterization of the isoenzymes of N-acetyl-β-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.",

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AU - Kimball, P. M.

AU - Brattain, M. G.

AU - White, W. E.

PY - 1981

Y1 - 1981

N2 - A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line. The separation and characterization of the isoenzymes of N-acetyl-β-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.

AB - A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line. The separation and characterization of the isoenzymes of N-acetyl-β-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.

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Characterization of an unusual isoenzyme of N-acetyl-β-D-hexosaminidase from a human colonic carcinoma cell line

Abstract

ASJC Scopus subject areas

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