Characterization of Antibodies to a Placenta‐Specific Antigen Cross‐Reacting With a Choriocarcinoma Cell Line

Subrata Sinha; Alok Bhattacharya; Abdul Hamid Band; Amitabh Gaur; L. M. Strivastava; G. P. Talwar

doi:10.1111/j.1600-0897.1984.tb00124.x

Characterization of Antibodies to a Placenta‐Specific Antigen Cross‐Reacting With a Choriocarcinoma Cell Line

Subrata Sinha, Alok Bhattacharya, Abdul Hamid Band, Amitabh Gaur, L. M. Strivastava, G. P. Talwar

Research output: Contribution to journal › Article

Abstract

ABSTRACT: Antibodies were raised in rabbits to purified human term placental villous plasma membrane. These were cytotoxic to human peripheral blood lymphocytes and manifested cross‐reactivity to kidney and liver. After absorption with these tissues, reactivity was retained with placental villous plasma membrane. The placental‐specific antiserum was fully reactive with BeWo choriocarcinoma cells, but was devoid of reaction with a human myeloma tumor cell line (GM2132). The antibodies were cytotoxic to BeWo in the presence of complement, as determined by trypan blue uptake, ⁵¹Cr release, and inhibition of uptake of ³H‐thymidine. 1984 Munksgaard

Original language	English (US)
Pages (from-to)	112-116
Number of pages	5
Journal	American Journal of Reproductive Immunology
Volume	6
Issue number	3
DOIs	https://doi.org/10.1111/j.1600-0897.1984.tb00124.x
State	Published - 1984

Keywords

Oncofetal antigens
choriocarcinoma
cytotoxic antibodies
placental plasma membrane

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Reproductive Medicine
Obstetrics and Gynecology

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Sinha, S., Bhattacharya, A., Band, A. H., Gaur, A., Strivastava, L. M., & Talwar, G. P. (1984). Characterization of Antibodies to a Placenta‐Specific Antigen Cross‐Reacting With a Choriocarcinoma Cell Line. American Journal of Reproductive Immunology, 6(3), 112-116. https://doi.org/10.1111/j.1600-0897.1984.tb00124.x

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AU - Strivastava, L. M.

AU - Talwar, G. P.

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AB - ABSTRACT: Antibodies were raised in rabbits to purified human term placental villous plasma membrane. These were cytotoxic to human peripheral blood lymphocytes and manifested cross‐reactivity to kidney and liver. After absorption with these tissues, reactivity was retained with placental villous plasma membrane. The placental‐specific antiserum was fully reactive with BeWo choriocarcinoma cells, but was devoid of reaction with a human myeloma tumor cell line (GM2132). The antibodies were cytotoxic to BeWo in the presence of complement, as determined by trypan blue uptake, 51Cr release, and inhibition of uptake of 3H‐thymidine. 1984 Munksgaard

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