Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs

Jimmy Kwang; Simon Yang; Fernando A. Osorio; Steve Christian; J. Galeota Wheeler; Kelly M. Lager; Sharon Low; Leo Chang; Alan R. Doster; Amy White; Ching Ching Wu

doi:10.1177/104063879901100501

Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs

Jimmy Kwang, Simon Yang, Fernando A. Osorio, Steve Christian, J. Galeota Wheeler, Kelly M. Lager, Sharon Low, Leo Chang, Alan R. Doster, Amy White, Ching Ching Wu

Research output: Contribution to journal › Review article › peer-review

3 Scopus citations

Abstract

The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.

Original language	English (US)
Pages (from-to)	391-395
Number of pages	5
Journal	Journal of Veterinary Diagnostic Investigation
Volume	11
Issue number	5
DOIs	https://doi.org/10.1177/104063879901100501
State	Published - Sep 1999

ASJC Scopus subject areas

veterinary(all)

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10.1177/104063879901100501

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Kwang, J., Yang, S., Osorio, F. A., Christian, S., Wheeler, J. G., Lager, K. M., Low, S., Chang, L., Doster, A. R., White, A., & Wu, C. C. (1999). Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs. Journal of Veterinary Diagnostic Investigation, 11(5), 391-395. https://doi.org/10.1177/104063879901100501

Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs. / Kwang, Jimmy; Yang, Simon; Osorio, Fernando A. et al.

In: Journal of Veterinary Diagnostic Investigation, Vol. 11, No. 5, 09.1999, p. 391-395.

Research output: Contribution to journal › Review article › peer-review

Kwang, J, Yang, S, Osorio, FA, Christian, S, Wheeler, JG, Lager, KM, Low, S, Chang, L, Doster, AR, White, A & Wu, CC 1999, 'Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs', Journal of Veterinary Diagnostic Investigation, vol. 11, no. 5, pp. 391-395. https://doi.org/10.1177/104063879901100501

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title = "Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs",

abstract = "The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.",

author = "Jimmy Kwang and Simon Yang and Osorio, {Fernando A.} and Steve Christian and Wheeler, {J. Galeota} and Lager, {Kelly M.} and Sharon Low and Leo Chang and Doster, {Alan R.} and Amy White and Wu, {Ching Ching}",

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AU - Yang, Simon

AU - Osorio, Fernando A.

AU - Christian, Steve

AU - Wheeler, J. Galeota

AU - Lager, Kelly M.

AU - Low, Sharon

AU - Chang, Leo

AU - Doster, Alan R.

AU - White, Amy

AU - Wu, Ching Ching

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Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs

Abstract

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