TY - JOUR
T1 - Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system
AU - Zhang, Yanping
AU - Nelson, Michael
AU - Nietfeldt, Joseph W.
AU - Burbank, Dwight E.
AU - van Etten, James L.
N1 - Funding Information:
We thank Geoff Wilson for helpful discussions. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences. This manuscript has been assigned Journal Series No. 10,049, Agricultural Research Division, University of Nebraska.
PY - 1992/10/25
Y1 - 1992/10/25
N2 - A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.
AB - A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.
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U2 - 10.1093/nar/20.20.5351
DO - 10.1093/nar/20.20.5351
M3 - Article
C2 - 1437552
AN - SCOPUS:0026707850
SN - 0305-1048
VL - 20
SP - 5351
EP - 5356
JO - Nucleic acids research
JF - Nucleic acids research
IS - 20
ER -