Characterization of DNA polymerases in an uninfected and virus PBCV-1-infected green alga - chlorella strain NC64A

Jan Rohozitiski, James L. Van Ellen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Chlorella NC64A cells infected with the large double-stranded DNA-contain-ing virus PBCV-1 and uninfected cells were assayed for DNA polymerase activity. Both uninfected and infected cells contained three forms of DNA polymerase activity: (i) an exogenous DNA-dependent 10,000 g soluble fraction, (ii) an exogenous DNA-dependent 10,000 g particulate fraction, and (iii) a DN A-independent 10,000 g particulate fraction. The three DNA polymerase activities in the infected and uninfected cells were distinguished from one another by the conditions required for optimum activity and by their sensitivity to inhibitors.

Original languageEnglish (US)
Pages (from-to)156-162
Number of pages7
JournalIntervirology
Volume30
Issue number3
DOIs
StatePublished - Jan 1 1989

Keywords

  • Algal viruses
  • Chlorella
  • DNA polymerases
  • PBCV-1

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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