@inproceedings{ef27f590e0aa4dc9ba4dae7ac81116ce,
title = "Characterization of DNA sequences that confer complement resistance in Leishmania chagasi",
abstract = "Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into high-passage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4) the sequence for one of the cosmids (Cosmid 51) was determined. This report extends the analysis of Cosmid 51, and identifies by deletion analysis a subregion of the cosmid insert that is critical to the CML-resistance phenotype of Cosmid 51 transformants. We also report the sequence determination and initial CML-resistance activity of another cosmid that also confers partial resistance to CML.",
keywords = "Complement, Immunity, Leishmania, Leishmania chagasi, Protozoa",
author = "Dahlin-Laborde, {Rebecca R.} and Scolaro, {Eric J.} and Romine, {Nathan M.} and Ramer-Tait, {Amanda E.} and Lei, {Soi Meng} and Beetham, {Jeffrey K.}",
year = "2008",
month = dec,
doi = "10.1196/annals.1428.099",
language = "English (US)",
isbn = "9781573317146",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "347--351",
booktitle = "Animal Biodiversity and Emerging Diseases Prediction and Prevention",
}