@article{119c9a3a20134b86a761204b74edd9ca,
title = "Characterization of gene expression regulated by human OTK18 using Drosophila melanogaster as a model system for innate immunity",
abstract = "OTK18 is a human transcriptional suppressor implicated in the regulation of human immunodeficiency virus type-one infection of mononuclear phagocytes. It is ubiquitously expressed in all normal tissues, but its normal homeostatic function is yet to be characterized. One hypothesis is that OTK18 aids in the regulation of the innate immune system. To test this hypothesis, cDNA microarray analysis was performed on the total RNA extracted from Drosophila melanogaster embryonic Schneider 2 (S2) cells transfected with either pEGFP-OTK18 (enhanced green fluorescent protein) or empty vector controls (pEGFP-N3) for 6, 12 and 24 h. cDNA microarray analysis revealed differential expression of genes known to be important in regulation of Drosophila innate immunity. The expression levels of two genes, Metchnikowin and CG16708 were verified by quantitative real-time reverse transcription PCR. These results suggest a role for OTK18 in innate immunity.",
keywords = "Drosophila melanogaster, Innate immunity, OTK18, S2 cells, cDNA microarray, qRT-PCR",
author = "Spresser, {Cole R.} and Marshall, {Sarah E.} and Carlson, {Kimberly A.}",
note = "Funding Information: We thank Dr Brad Ericson and Mr Darby Carlson for their editorial and technical support. Dr Paul Twigg, Ms Anjeza Pashaj and Ms Teresa Donze from the University of Nebraska at Kearney (UNK), and Dr Lucy Cherbas of the DGRC for technical support. Dr Julie Shaffer for reviewing the manuscript. Dr Jim Eudy of the UNMC Microarray Core facility for performing the cDNA microarray analysis. Dr Lynette Smith of the UNMC Department of Preventive and Societal Medicine, for performing the statistical analyses. A kind gift of pEGFP-OTK18 received from Dr Tsuneya Ikezu of the Department of Pharmacology and Experimental Neuroscience at UNMC. This publication was made possible by NIH grant number P20 RR016469 from the INBRE Program of the National Center for Research Resources, UNK Research Services Council Mini and Creative Activity Grants, UNK Office of Sponsored Programmes (Summer Student Research Programme), UNK College of Natural and Social Sciences and the UNK Biology Department.",
year = "2008",
month = aug,
doi = "10.1007/s12041-008-0017-3",
language = "English (US)",
volume = "87",
pages = "109--117",
journal = "Journal of Genetics",
issn = "0022-1333",
publisher = "Springer",
number = "2",
}