Characterization of human colon carcinoma cell lines isolated from a single primary tumour

M. G. Brattain, M. E. Marks, J. McCombs, W. Finely, D. E. Brattain

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.

Original languageEnglish (US)
Pages (from-to)373-381
Number of pages9
JournalBritish journal of cancer
Issue number3
StatePublished - Mar 1983

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


Dive into the research topics of 'Characterization of human colon carcinoma cell lines isolated from a single primary tumour'. Together they form a unique fingerprint.

Cite this