Characterization of human colon carcinoma cell lines isolated from a single primary tumour

M. G. Brattain, M. E. Marks, J. McCombs, W. Finely, D. E. Brattain

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59 Scopus citations

Abstract

The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.

Original languageEnglish (US)
Pages (from-to)373-381
Number of pages9
JournalBritish journal of cancer
Volume47
Issue number3
DOIs
StatePublished - Mar 1983
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Brattain, M. G., Marks, M. E., McCombs, J., Finely, W., & Brattain, D. E. (1983). Characterization of human colon carcinoma cell lines isolated from a single primary tumour. British journal of cancer, 47(3), 373-381. https://doi.org/10.1038/bjc.1983.56