Characterization of Mahogunin Ring Finger-1 expression in mice

Pooneh Bagher, Jian Jiao, C. Owen Smith, Christina D. Cota, Teresa M. Gunn

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Mutations in mouse Mahogunin Ring Finger-1 (Mgrn1) were first recognized for their effect on agouti-mediated pigment-type switching. Mgrn1 null mutants are completely black and develop spongiform degeneration of the brain. Mgrn1 hypomorphs have dark fur but do not develop neurodegeneration. We characterized a new Mgrn1 hypomorphic allele caused by a gene-trap insertion. Mice homozygous for this mutation are slightly darker than non-mutant animals. They show reduced overall expression of Mgrn1 and two of the four normal Mgrn1 isoforms are replaced by β-GEO fusion proteins that differ from the normal proteins at their carboxy termini. To investigate the role of different Mgrn1 isoforms in pigment-type switching, we used quantitative relative reverse transcriptase polymerase chain reaction to examine their expression in the skin of Mgrn1 mutant and control mice. Most Mgrn1 mutants produce little or no normal Mgrn1 in the skin. Mgrn1 null mutant mice overexpressing isoform I or III, which are normally absent or weakly expressed in adult skin, had normal agouti-banded hairs. Our results indicate that reduced levels of MGRN1 cause the pigmentation phenotypes of Mgrn1 mutant mice and that there are no significant differences in the function of the four MGRN1 isoforms in pigment-type switching.

Original languageEnglish (US)
Pages (from-to)635-643
Number of pages9
JournalPigment Cell Research
Issue number6
StatePublished - Dec 2006
Externally publishedYes


  • Agouti
  • Mahoganoid
  • Mahogunin Ring Finger-1
  • Mouse
  • Pigmentation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'Characterization of Mahogunin Ring Finger-1 expression in mice'. Together they form a unique fingerprint.

Cite this