Characterization of metastasis-associated antigens on RAW117 lymphosarcoma cell lines

Shantaram S. Joshi, John G. Sharp, Hemant M. Gharpure, Kenneth W. Brunson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces. Antigen-I was uniformly dsitributed ov er the surface of these cells wheras antigen-II had a patehy, punctate distribution, Antigen-I was displayed less on RAW1 17-1110 cells than on RAW117-P cells, while the expression of the other serologically distinct antigen (antigen-II) was increased on RAW117-H10 cells compared to the less malignant parental (RAW117-P) cells. This differential antigen expression was assessed by immunodiffusion, a 125I-labeled protein-A binding assay, flow cytometry and rocket immunoelectrophoresis. Both these antigens had a molecular weight of 70000 daltons. Antigen-I bound the lectin concanavalin-A whereas antigen- II did not, suggesting that antigen-I might be the viral envelope glycoprotein gp70. The identity of antigen-II is presently unknown. Syngeneic Balb/c mice injected with highly malignant and metastatic RAW117-H10 cells coated with antiserum to antigen-I were protected from early death; this effect was mot seem with RAW1 17-1110 cells coated with amtoseri, to antigen-II. The opsonizing qualities of thes antisera may be different due to antibody to antigen-II being shed more rapidly than antibody to antigen-I.

Original languageEnglish (US)
Pages (from-to)89-104
Number of pages16
JournalClinical & Experimental Metastasis
Volume5
Issue number1
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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