A syngeneic murine model system was used to study the immunobiology of metastasis. The highly malignant RAW117-H10 cell line was compared to the less malignant parental RAW117-P cell line from which it was derived, for expression of cell surface antigens. Using rabbit antisera, two major glycoprotein antigens were detected on the tumor cell surfaces. Antigen-I was uniformly dsitributed ov er the surface of these cells wheras antigen-II had a patehy, punctate distribution, Antigen-I was displayed less on RAW1 17-1110 cells than on RAW117-P cells, while the expression of the other serologically distinct antigen (antigen-II) was increased on RAW117-H10 cells compared to the less malignant parental (RAW117-P) cells. This differential antigen expression was assessed by immunodiffusion, a 125I-labeled protein-A binding assay, flow cytometry and rocket immunoelectrophoresis. Both these antigens had a molecular weight of 70000 daltons. Antigen-I bound the lectin concanavalin-A whereas antigen- II did not, suggesting that antigen-I might be the viral envelope glycoprotein gp70. The identity of antigen-II is presently unknown. Syngeneic Balb/c mice injected with highly malignant and metastatic RAW117-H10 cells coated with antiserum to antigen-I were protected from early death; this effect was mot seem with RAW1 17-1110 cells coated with amtoseri, to antigen-II. The opsonizing qualities of thes antisera may be different due to antibody to antigen-II being shed more rapidly than antibody to antigen-I.
ASJC Scopus subject areas
- Cancer Research