Characterization of peptide YY receptors in rabbit colonic mucosa

P. J. Mannon, E. J. Hernandez, S. J. Mervin, S. R. Vigna, I. L. Taylor

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY ≥ neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.

Original languageEnglish (US)
Pages (from-to)567-572
Number of pages6
JournalPeptides
Volume14
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • Colon
  • Peptide YY
  • Receptor

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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