Characterization of peptide YY receptors in rabbit colonic mucosa

P. J. Mannon; E. J. Hernandez; S. J. Mervin; S. R. Vigna; I. L. Taylor

doi:10.1016/0196-9781(93)90146-8

Characterization of peptide YY receptors in rabbit colonic mucosa

P. J. Mannon, E. J. Hernandez, S. J. Mervin, S. R. Vigna, I. L. Taylor

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Quantitative receptor autoradiography localized a high-affinity binding site for ¹²⁵I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (K_D = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY ≥ neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (K_D = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these ¹²⁵I-peptide YY binding sites to resemble the Y₁-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.

Original language	English (US)
Pages (from-to)	567-572
Number of pages	6
Journal	Peptides
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(93)90146-8
State	Published - 1993
Externally published	Yes

Keywords

Colon
Peptide YY
Receptor

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(93)90146-8

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title = "Characterization of peptide YY receptors in rabbit colonic mucosa",

abstract = "Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY ≥ neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.",

keywords = "Colon, Peptide YY, Receptor",

author = "Mannon, {P. J.} and Hernandez, {E. J.} and Mervin, {S. J.} and Vigna, {S. R.} and Taylor, {I. L.}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by the Department of Veterans Affairs (P.J.M.) and NIH grant DK 38216 (I.L.T.).",

year = "1993",

doi = "10.1016/0196-9781(93)90146-8",

language = "English (US)",

volume = "14",

pages = "567--572",

journal = "Peptides",

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number = "3",

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T1 - Characterization of peptide YY receptors in rabbit colonic mucosa

AU - Mannon, P. J.

AU - Hernandez, E. J.

AU - Mervin, S. J.

AU - Vigna, S. R.

AU - Taylor, I. L.

N1 - Funding Information: ACKNOWLEDGEMENTS This work was supported by the Department of Veterans Affairs (P.J.M.) and NIH grant DK 38216 (I.L.T.).

PY - 1993

Y1 - 1993

N2 - Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY ≥ neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.

AB - Quantitative receptor autoradiography localized a high-affinity binding site for 125I-peptide YY to the mucosa in rabbit distal colon. Scatchard binding analysis revealed a single-affinity binding site (KD = 0.29 nM) with binding specificity similar to other peptide YY-preferring receptors (peptide YY ≥ neuropeptide Y > pancreatic polypeptide). Radioligand binding studies using colonic mucosal membranes confirmed high-affinity peptide YY binding sites (KD = 0.26 nM) with time, temperature, and protein dependence, as well as saturability characteristic of receptor-ligand binding. Selective peptide analogues showed a subpopulation of these 125I-peptide YY binding sites to resemble the Y1-type neuropeptide Y receptor. Peptide YY may exert local antisecretory effects on the colonic epithelium via these binding sites.

KW - Colon

KW - Peptide YY

KW - Receptor

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DO - 10.1016/0196-9781(93)90146-8

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AN - SCOPUS:0027274009

SN - 0196-9781

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SP - 567

EP - 572

JO - Peptides

JF - Peptides

IS - 3

ER -

Characterization of peptide YY receptors in rabbit colonic mucosa

Abstract

Keywords

ASJC Scopus subject areas

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