Characterization of Soybean Events with Enhanced Expression of the Microtubule-Associated Protein 65-1 (MAP65-1)

Panya Kim, Samira Mahboob, Hanh T. Nguyen, Samuel Eastman, Olivia Fiala, Matthew Sousek, Roch E. Gaussoin, Jae L. Brungardt, Tamra A. Jackson-Ziems, Rebecca Roston, James R. Alfano, Tom Elmo Clemente, Ming Guo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Microtubule-associated protein 65-1 (MAP65-1) protein plays an essential role in plant cellular dynamics through impacting stabilization of the cytoskeleton by serving as a crosslinker of microtubules. The role of MAP65-1 in plants has been associated with phenotypic outcomes in response to various environmental stresses. The Arabidopsis MAP65-1 (AtMAP65-1) is a known virulence target of plant bacterial pathogens and is thus a component of plant immunity. Soybean events were generated that carry transgenic alleles for both AtMAP65-1 and GmMAP65-1, the soybean AtMAP65-1 homolog, under control of cauliflower mosaic virus 35S promoter. Both AtMAP65-1 and GmMAP65-1 transgenic soybeans are more resistant to challenges by the soybean bacterial pathogen Pseudomonas syringae pv. glycinea and the oomycete pathogen Phytophthora sojae, but not the soybean cyst nematode, Heterodera glycines. Soybean plants expressing AtMAP65-1 and GmMAP65-1 also display a tolerance to the herbicide oryzalin, which has a mode of action to destabilize microtubules. In addition, GmMAP65-1-expressing soybean plants show reduced cytosol ion leakage under freezing conditions, hinting that ectopic expression of GmMAP65-1 may enhance cold tolerance in soybean. Taken together, overexpression of AtMAP65-1 and GmMAP65-1 confers tolerance of soybean plants to various biotic and abiotic stresses.

Original languageEnglish (US)
Pages (from-to)62-71
Number of pages10
JournalMolecular Plant-Microbe Interactions
Issue number1
StatePublished - Jan 2024


  • bacterial pathogenesis
  • nematode–plant interactions
  • oomycete–plant interactions

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science


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