Characterization of species C human adenovirus serotype 6 (Ad6)

Eric A. Weaver, Mathew L. Hillestad, Reeti Khare, Donna Palmer, Philip Ng, Michael A. Barry

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than the others which may explain differences in virus infectivity in vitro, but not in the liver. Comparison of hexon hypervariable regions (HVRs) suggests that the higher transduction by Ad5 and 6 as compared to Ad1 and 2 may be related to differences in charge and length.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalVirology
Volume412
Issue number1
DOIs
StatePublished - Mar 30 2011

Keywords

  • Adenovirus
  • Fiber
  • Hexon
  • Liver infection
  • Sequence
  • Species C

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Characterization of species C human adenovirus serotype 6 (Ad6)'. Together they form a unique fingerprint.

Cite this