Characterization of the ATPase activity of RecG and RuvAB proteins on model fork structures reveals insight into stalled DNA replication fork repair

Syafiq Abd Wahab, Meerim Choi, Piero R. Bianco

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Background: DNA replication fork rescue requires the action of DNA helicases to regress the fork. Results: RecG is more active than RuvAB on substrates that mimic nascent stalled forks, whereas RuvAB is active on Holliday junctions. Conclusion: RecG in concert with SSB regresses stalledDNAreplication forks, producingDNAsubstrates to which RuvAB can bind. Significance: RecG, not RuvAB, regresses stalled DNA replication forks.

Original languageEnglish (US)
Pages (from-to)26397-26409
Number of pages13
JournalJournal of Biological Chemistry
Volume288
Issue number37
DOIs
StatePublished - Sep 13 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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