TY - JOUR
T1 - Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs
AU - Carrington, J. C.
AU - Morris, T. J.
N1 - Funding Information:
The authors thank Mr. Bradley Hillman for helpful suggestions concerning this work and manuscript, Mr. Drake Stenger for generously supplying antisera, and Vivian Tung for excellent typing. This work was supported in part by Grant 8%CRCR-l-1227 from the U. S. Department of Agriculture.
PY - 1985/7/15
Y1 - 1985/7/15
N2 - The in vitro translation products of carnation mottle virus (CarMV) genomic and subgenomic RNAs were analysed using a rabbit reticulocyte lysate system. Viral RNAs directed synthesis of three main polypeptides, p80, p40, and p34. p40, which was the predominant product using unfractionated virion RNA as template, was identified as coat protein based on electrophoretic mobility through SDS-polyacrylamide gels and immunoprecipitation with anti-CarMV serum. Upon size-fractionation of virion RNAs by sucrose gradient centrifugation and subsequent translational analysis, p40 was found to be encoded by subgenomic RNA, whereas p80 and p34 were synthesized from templates of genome length. p80 and p34 were found to contain common or overlapping amino acid sequences by analysis of cleavage peptides formed during proteolysis with α-chymotrypsin. These data support CarMV translational mechanisms other than those proposed in a previous study (R. Saloman, M. Bar-Joseph, H. Soreq, I. Gozes, and U. Z. Littauer, 1978,Virology 90, 288-298).
AB - The in vitro translation products of carnation mottle virus (CarMV) genomic and subgenomic RNAs were analysed using a rabbit reticulocyte lysate system. Viral RNAs directed synthesis of three main polypeptides, p80, p40, and p34. p40, which was the predominant product using unfractionated virion RNA as template, was identified as coat protein based on electrophoretic mobility through SDS-polyacrylamide gels and immunoprecipitation with anti-CarMV serum. Upon size-fractionation of virion RNAs by sucrose gradient centrifugation and subsequent translational analysis, p40 was found to be encoded by subgenomic RNA, whereas p80 and p34 were synthesized from templates of genome length. p80 and p34 were found to contain common or overlapping amino acid sequences by analysis of cleavage peptides formed during proteolysis with α-chymotrypsin. These data support CarMV translational mechanisms other than those proposed in a previous study (R. Saloman, M. Bar-Joseph, H. Soreq, I. Gozes, and U. Z. Littauer, 1978,Virology 90, 288-298).
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U2 - 10.1016/0042-6822(85)90299-5
DO - 10.1016/0042-6822(85)90299-5
M3 - Article
C2 - 18640513
AN - SCOPUS:0021933810
VL - 144
SP - 1
EP - 10
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -