Characterization of the maize xylem sap proteome

Sophie Alvarez; Jason Q.D. Goodger; Ellen L. Marsh; Sixue Chen; Victor S. Asirvatham; Daniel P. Schachtman

doi:10.1021/pr050471q

Characterization of the maize xylem sap proteome

Sophie Alvarez, Jason Q.D. Goodger, Ellen L. Marsh, Sixue Chen, Victor S. Asirvatham, Daniel P. Schachtman

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.

Original language	English (US)
Pages (from-to)	963-972
Number of pages	10
Journal	Journal of proteome research
Volume	5
Issue number	4
DOIs	https://doi.org/10.1021/pr050471q
State	Published - Apr 2006
Externally published	Yes

Keywords

Antifungal activity
Corn
Glycoprotein
Isoform
Maize
Plants
Proteome
Xylem sap

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

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10.1021/pr050471q

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abstract = "The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.",

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T1 - Characterization of the maize xylem sap proteome

AU - Alvarez, Sophie

AU - Goodger, Jason Q.D.

AU - Marsh, Ellen L.

AU - Chen, Sixue

AU - Asirvatham, Victor S.

AU - Schachtman, Daniel P.

PY - 2006/4

Y1 - 2006/4

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AB - The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.

KW - Antifungal activity

KW - Corn

KW - Glycoprotein

KW - Isoform

KW - Maize

KW - Plants

KW - Proteome

KW - Xylem sap

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Characterization of the maize xylem sap proteome

Abstract

Keywords

ASJC Scopus subject areas

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