Characterization of the soluble guanylyl cyclase β-subunit gene in the mosquito Anopheles gambiae

Adalgisa Caccone, B. A. Garcia, K. D. Mathiopoulos, G. S. Min, E. N. Moriyama, J. R. Powell

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Genomic DNA corresponding to the soluble guanylyl cyclase β-subunit (GCSβ) gene was cloned and sequenced from Anopheles gambiae. The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71% and 62% similar to previously known Drosophila and vertebrate GCSβ, while the C-terminus of A. gambiae GCSβ was shorter. Because of the conserved characteristics in each functional domain, the high G+C% in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron-exon boundaries). Interestingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus-27 transposable element and other noncoding regions of the A. gambiae genome.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalInsect Molecular Biology
Issue number1
StatePublished - Feb 1999
Externally publishedYes


  • Anopheles gambiae
  • DNA
  • Guanylyl cyclase β-subunit
  • Molecular evolution
  • Pegasus transposable element

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science


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