Abstract
Genomic DNA corresponding to the soluble guanylyl cyclase β-subunit (GCSβ) gene was cloned and sequenced from Anopheles gambiae. The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71% and 62% similar to previously known Drosophila and vertebrate GCSβ, while the C-terminus of A. gambiae GCSβ was shorter. Because of the conserved characteristics in each functional domain, the high G+C% in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron-exon boundaries). Interestingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus-27 transposable element and other noncoding regions of the A. gambiae genome.
Original language | English (US) |
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Pages (from-to) | 23-30 |
Number of pages | 8 |
Journal | Insect Molecular Biology |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1999 |
Externally published | Yes |
Keywords
- Anopheles gambiae
- DNA
- Guanylyl cyclase β-subunit
- Molecular evolution
- Pegasus transposable element
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Insect Science