TY - JOUR
T1 - Characterization of two chitinase genes and one chitosanase gene encoded by chlorella virus PBCV-1
AU - Sun, Liangwu
AU - Adams, Byron
AU - Gurnon, James R.
AU - Ye, Yen
AU - Van Etten, James L.
N1 - Funding Information:
We thank Les Lane, Michael Graves, Mike Nelson, and Zhongge Zhang for helpful discussions and Steven Jones for use of his fluorimeter. This investigation was supported, in part, by Public Health Service Grant GM-32441, NSF-EPSCoR Cooperative Agreement EPS-9255225, and an AOC grant from the University of Nebraska Biotechnology Center. This paper has been assigned Journal Series No. 12696, Agricultural Research Division, University of Nebraska.
PY - 1999/10/25
Y1 - 1999/10/25
N2 - Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a2921. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recombinant A292L has chitosanase activity. Transcription of a181/182r, a260r, and a2921 genes begins at 30, 60, and 60 min p.i., respectively; transcription of all three genes continues until the cells lyse. A181/182R, A260R, and A292L proteins are first detected by Western blots at 60, 90, and 120 min p.i., respectively. Therefore, a181/182r is an early gene and a260r and a2921 are late genes. All three genes are widespread in chlorella viruses. Phylogenetic analyses indicate that the ancestral condition of the a 181/182r gene arose from the most recent common ancestor of a gene found in tobacco, whereas the genealogical position of the a260r gene could not be unambiguously resolved.
AB - Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a2921. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recombinant A292L has chitosanase activity. Transcription of a181/182r, a260r, and a2921 genes begins at 30, 60, and 60 min p.i., respectively; transcription of all three genes continues until the cells lyse. A181/182R, A260R, and A292L proteins are first detected by Western blots at 60, 90, and 120 min p.i., respectively. Therefore, a181/182r is an early gene and a260r and a2921 are late genes. All three genes are widespread in chlorella viruses. Phylogenetic analyses indicate that the ancestral condition of the a 181/182r gene arose from the most recent common ancestor of a gene found in tobacco, whereas the genealogical position of the a260r gene could not be unambiguously resolved.
UR - http://www.scopus.com/inward/record.url?scp=0033604280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033604280&partnerID=8YFLogxK
U2 - 10.1006/viro.1999.9958
DO - 10.1006/viro.1999.9958
M3 - Article
C2 - 10544110
AN - SCOPUS:0033604280
VL - 263
SP - 376
EP - 387
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -