TY - JOUR
T1 - Characterization of viruses infecting a eukaryotic Chlorella-like green alga
AU - Schuster, Anne M.
AU - Burbank, Dwight E.
AU - Meister, Barbara
AU - Skrdla, Merri P.
AU - Meints, Russel H.
AU - Hattman, Stanley
AU - Swinton, David
AU - Van Etten, James L.
N1 - Funding Information:
We thank Myron Brakke and Les Lane for many helpful discussions. This investigation was supported, in part, by Public Health Services Grants GM-32441 (JVE) and GM 2922’7 (SH) from the National Institute of General Medical Sciences and Grant DE-ACOL 82ERl2086 (JVE) from the Department of Energy.
PY - 1986/4/15
Y1 - 1986/4/15
N2 - Nineteen plaque-forming viruses of the unicellular, eukaryotic Chlorella-like green alga, strain NC64A, were isolated from various geographic regions in the United States and characterized. Like the previously described virus, PBCV-1, all of the new viruses were large polyhedrons, sensitive to chloroform, and contained large dsDNA genomes of ca. 300 kbp. All of the viral DNAs contained 5-methyldeoxycytidine which varied from 0.1 to 47% of the deoxycytidine. In addition, 10 of the viral DNAs contained N6-methyldeoxyadenosine which varied from 8.1 to 37% of the deoxyadenosine. These viruses, along with 11 previously described viruses which replicate in the same Chlorella host, were grouped into 11 classes based on at least one of the following properties: plaque size, reaction with PBCV-1 antiserum, or the nature and abundance of methylated bases in their genomic DNA.
AB - Nineteen plaque-forming viruses of the unicellular, eukaryotic Chlorella-like green alga, strain NC64A, were isolated from various geographic regions in the United States and characterized. Like the previously described virus, PBCV-1, all of the new viruses were large polyhedrons, sensitive to chloroform, and contained large dsDNA genomes of ca. 300 kbp. All of the viral DNAs contained 5-methyldeoxycytidine which varied from 0.1 to 47% of the deoxycytidine. In addition, 10 of the viral DNAs contained N6-methyldeoxyadenosine which varied from 8.1 to 37% of the deoxyadenosine. These viruses, along with 11 previously described viruses which replicate in the same Chlorella host, were grouped into 11 classes based on at least one of the following properties: plaque size, reaction with PBCV-1 antiserum, or the nature and abundance of methylated bases in their genomic DNA.
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U2 - 10.1016/0042-6822(86)90276-X
DO - 10.1016/0042-6822(86)90276-X
M3 - Article
C2 - 3006334
AN - SCOPUS:0022641763
SN - 0042-6822
VL - 150
SP - 170
EP - 177
JO - Virology
JF - Virology
IS - 1
ER -