Chlamydiazyme plus blocking assay to detect Chlamydia trachomatis in endocervical specimens

R. D. Mills, A. Young, K. Cain, T. M.H. Blair, M. A. Sitorius, G. L. Woods

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Three methods to detect Chlamydia trachomatis in endocervical swab specimens collected from 502 women with genitourinary or abdominopelvic symptoms were evaluated: (1) a direct immunofluorescence assay, (2) an enzyme-linked immunoabsorbent assay, confirming positive samples with a blocking assay, and (3) conventional tissue cell culture. C. trachomatis was detected by at least one method in 72 specimens, of which 56 (11%) were determined to be true-positive results by repeated testing and by performing a confirmatory assay. The sensitivity, specificity, and positive and negative predictive values were 91%, 100%, 100%, and 99%, respectively, for culture and the enzyme-linked immunoabsorbent assay plus blocking assay and 74%, 98%, 83%, and 96%, respectively, for the direct immunofluorescence assay. In this population of women, using the enzyme-linked immunoabsorbent assay with the confirmatory assay is a rapid, reliable, and cost-effective alternative to culture for diagnosing infection with C. trachomatis.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalAmerican journal of clinical pathology
Issue number2
StatePublished - 1992


  • Blocking assay
  • Chlamydia trachomatis
  • Chlamydiazyme
  • Enzyme-linked immunoassay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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