TY - JOUR
T1 - Chlorella virus NY-2A encodes at least 12 DNA endonuclease/methyltransferase genes
AU - Zhang, Yanping
AU - Nelson, Michael
AU - Nietfeldt, Joe
AU - Xia, Yuannan
AU - Burbank, Dwight
AU - Ropp, Susan
AU - Van Etten, James L.
N1 - Funding Information:
We thank Kathryn Maney for technical assistance. This investigation was supported, in part, by Public Health Service Grant GM-32441 to J.V.E. and NIGMS Grant R4GM48909B to Megabase Research Products.
PY - 1998/1/15
Y1 - 1998/1/15
N2 - The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6- methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GT(m)AC), M.CvQII (R(m)AR), M.CviQIII (TCG(m)A), M.CviQIV (G(m)ATC), M.CviQV (TGC(m)A), M.CviQVI (G(m)ANTC), and M.CviQVII (C(m)ATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RG(m)C(T/C/G)], M.CviQIX ((m)CC), and M.CviQX ((m)CGR). Five of the 6mA methyltransferase genes, M.CviQI, M. CviQIII, M. CviQV, M.CviQVI, and M.CviQVII, were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY- 2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3-4% of the virus genome.
AB - The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6- methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GT(m)AC), M.CvQII (R(m)AR), M.CviQIII (TCG(m)A), M.CviQIV (G(m)ATC), M.CviQV (TGC(m)A), M.CviQVI (G(m)ANTC), and M.CviQVII (C(m)ATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RG(m)C(T/C/G)], M.CviQIX ((m)CC), and M.CviQX ((m)CGR). Five of the 6mA methyltransferase genes, M.CviQI, M. CviQIII, M. CviQV, M.CviQVI, and M.CviQVII, were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY- 2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3-4% of the virus genome.
UR - http://www.scopus.com/inward/record.url?scp=0032518484&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032518484&partnerID=8YFLogxK
U2 - 10.1006/viro.1997.8936
DO - 10.1006/viro.1997.8936
M3 - Article
C2 - 9454710
AN - SCOPUS:0032518484
SN - 0042-6822
VL - 240
SP - 366
EP - 375
JO - Virology
JF - Virology
IS - 2
ER -