TY - JOUR
T1 - Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases
AU - Que, Qiudeng
AU - Zhang, Yanping
AU - Nelson, Michael
AU - Ropp, Susan
AU - Burbank, Dwight E.
AU - Van Etten, James L.
N1 - Funding Information:
This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences.
PY - 1997
Y1 - 1997
N2 - Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.
AB - Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.
KW - 5-Methyldeoxycytosine methyltransferase
KW - 5-Methyldeoxycytosine methyltransferase pseudogene
KW - N-methyldeoxyadenine methyltransferase
KW - Recombinant DNA
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U2 - 10.1016/S0378-1119(96)00862-1
DO - 10.1016/S0378-1119(96)00862-1
M3 - Article
C2 - 9197539
AN - SCOPUS:0030908254
VL - 190
SP - 237
EP - 244
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -