Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases

Qiudeng Que; Yanping Zhang; Michael Nelson; Susan Ropp; Dwight E. Burbank; James L. Van Etten

doi:10.1016/S0378-1119(96)00862-1

Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases

Qiudeng Que, Yanping Zhang, Michael Nelson, Susan Ropp, Dwight E. Burbank, James L. Van Etten

Plant Pathology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N⁶-methyldeoxyadenine (m⁶A) MTases, M·CviSI (TGC^mA), M·CviSII (C^mATG), M·CviSIII (TCG^mA) and M·CviSIV (G^mATC), one 5-methyldeoxycytosine (m⁵C) MTase, M·CviSV (~ RC^mCG), and one nonfunctional m⁵C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RG^mC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m⁶A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m⁵C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m⁵C MTase. The three cloned m⁶A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.

Original language	English (US)
Pages (from-to)	237-244
Number of pages	8
Journal	Gene
Volume	190
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(96)00862-1
State	Published - 1997

Keywords

5-Methyldeoxycytosine methyltransferase
5-Methyldeoxycytosine methyltransferase pseudogene
N-methyldeoxyadenine methyltransferase
Recombinant DNA

ASJC Scopus subject areas

Genetics

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@article{0a22db59704a49afa1a88cda41616d47,

title = "Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases",

abstract = "Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.",

keywords = "5-Methyldeoxycytosine methyltransferase, 5-Methyldeoxycytosine methyltransferase pseudogene, N-methyldeoxyadenine methyltransferase, Recombinant DNA",

author = "Qiudeng Que and Yanping Zhang and Michael Nelson and Susan Ropp and Burbank, {Dwight E.} and {Van Etten}, {James L.}",

year = "1997",

doi = "10.1016/S0378-1119(96)00862-1",

language = "English (US)",

volume = "190",

pages = "237--244",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

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TY - JOUR

T1 - Chlorella virus SC-1A encodes at least five functional and one nonfunctional DNA methyltransferases

AU - Que, Qiudeng

AU - Zhang, Yanping

AU - Nelson, Michael

AU - Ropp, Susan

AU - Burbank, Dwight E.

AU - Van Etten, James L.

PY - 1997

Y1 - 1997

N2 - Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.

AB - Chlorella virus SC-1A encodes at least six DNA methyltransferases (MTases): four N6-methyldeoxyadenine (m6A) MTases, M·CviSI (TGCmA), M·CviSII (CmATG), M·CviSIII (TCGmA) and M·CviSIV (GmATC), one 5-methyldeoxycytosine (m5C) MTase, M·CviSV (~ RCmCG), and one nonfunctional m5C MTase, M·CviSVI, which is homologous to the MTase M·CviJI [RGmC(T/C/G)] produced by another chlorella virus IL-3A. Genes encoding three of the SC-1A m6A MTases (M·CviSI, M·CviSII, and M·CviSIII) and the nonfunctional m5C MTase were cloned and sequenced. Neither M·CviSI nor M·CviSIII genes hybridized to genes for their respective isomethylomers, M·CviRI and M·CviBIII, from other chlorella viruses. However, the M·CviSII gene hybridized strongly to its M·CviAII isomethylomer gene from virus PBCV-1. Like the prototype chlorella virus PBCV-1, the SC-1A genome contains inverted terminal repeats, one of which is adjacent to the nonfunctional m5C MTase. The three cloned m6A MTase genes are distributed throughout the approx. 345 kb SC-1A genome.

KW - 5-Methyldeoxycytosine methyltransferase

KW - 5-Methyldeoxycytosine methyltransferase pseudogene

KW - N-methyldeoxyadenine methyltransferase

KW - Recombinant DNA

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U2 - 10.1016/S0378-1119(96)00862-1

DO - 10.1016/S0378-1119(96)00862-1

M3 - Article

C2 - 9197539

AN - SCOPUS:0030908254

VL - 190

SP - 237

EP - 244

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -