TY - JOUR
T1 - Chloride-bicarbonate exchangers in the human fetal pancreas
AU - Hyde, Karen
AU - Harrison, Deborah
AU - Hollingsworth, Michael A.
AU - Harris, Ann
N1 - Funding Information:
Grant support: DK46589 from the National Institutes of Health, U.S.A., and the Cystic Fibrosis Trust, UK. 1Current address: Nuffield Department of Surgery, Oxford University. 2To whom correspondence should be addressed. Fax: 44-1865-222626. E-mail: [email protected].
PY - 1999/9/24
Y1 - 1999/9/24
N2 - Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes a small conductance cAMP-activated chloride ion channel. In the CF pancreatic duct, mutations in CFTR cause a reduction in bicarbonate secretion. This is thought to result from CFTR operating in parallel with a chloride-bicarbonate (Cl-/HCO3-) exchanger, located in the apical membrane of pancreatic duct cells. The molecular basis of this Cl-/HCO3- exchanger has not been identified. A combination of screening cDNA libraries, RNase protection, and 5' RACE analysis was used to identify Cl-/HCO3- exchangers in human fetal pancreas. An AE2 Cl-/HCO3- exchanger was shown to be expressed in human fetal pancreas from the midtrimester of gestation, at a time when CF-associated pathology commences. In addition, an AE1 Cl-/HCO3- was identified in fetal pancreas but was absent from the adult pancreas and cultured ductal epithelial cells from fetal and adult pancreas.
AB - Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that encodes a small conductance cAMP-activated chloride ion channel. In the CF pancreatic duct, mutations in CFTR cause a reduction in bicarbonate secretion. This is thought to result from CFTR operating in parallel with a chloride-bicarbonate (Cl-/HCO3-) exchanger, located in the apical membrane of pancreatic duct cells. The molecular basis of this Cl-/HCO3- exchanger has not been identified. A combination of screening cDNA libraries, RNase protection, and 5' RACE analysis was used to identify Cl-/HCO3- exchangers in human fetal pancreas. An AE2 Cl-/HCO3- exchanger was shown to be expressed in human fetal pancreas from the midtrimester of gestation, at a time when CF-associated pathology commences. In addition, an AE1 Cl-/HCO3- was identified in fetal pancreas but was absent from the adult pancreas and cultured ductal epithelial cells from fetal and adult pancreas.
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U2 - 10.1006/bbrc.1999.1367
DO - 10.1006/bbrc.1999.1367
M3 - Article
C2 - 10491290
AN - SCOPUS:0033600890
SN - 0006-291X
VL - 263
SP - 315
EP - 321
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -