Chlorinated benzothiadiazines inhibit angiogenesis through suppression of VEGFR2 phosphorylation

Bader I. Huwaimel, Sravan Jonnalagadda, Shirisha Jonnalagadda, Fatema T. Zahra, Alessio Nocentini, Claudiu T. Supuran, Constantinos M. Mikelis, Paul C. Trippier

Research output: Contribution to journalArticlepeer-review

Abstract

Angiogenesis inhibitors are a critical pharmacological tool for the treatment of solid tumors. Suppressing vascular permeability leads to inhibition of tumor growth, invasion, and metastatic potential by blocking the supply of oxygen and nutrients. Disruption of the vascular endothelial growth factor (VEGF) signaling pathway is a validated target for the design of antiangiogenic agents. Several VEGFR2 inhibitors have been clinically approved over the past years. Structural analysis of these clinical VEGFR2 inhibitors highlighted key functional group overlap with the benzothiadiazine core contained in a library of in-house compounds. Herein we ascribe anti-angiogenic activity to a series of chlorinated benzothiadiazines. Selected compounds show significant activity to completely ameliorate VEGF-induced endothelial cell proliferation by suppression of VEGFR2 phosphorylation. The scaffold is devoid of activity to inhibit carbonic anhydrases and generally lacks cytotoxicity across a range of cancer and non-malignant cell lines. Assay of activity at 468 kinases shows remarkable selectivity with only four kinases inhibited > 65% at 10 µM concentration, and with significant activity to inhibit TNK2/ACK1 and PKRD2 by > 90%. All four identified kinase targets are known modulators of angiogenesis, thus highlighting compound 17b as a novel angiogenesis inhibitor for further development.

Original languageEnglish (US)
Article number116805
JournalBioorganic and Medicinal Chemistry
Volume67
DOIs
StatePublished - Aug 1 2022

Keywords

  • Angiogenesis
  • Kinase inhibitor
  • Mechanism of action
  • VEGFR2

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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