Chromatin imaging with time-lapse atomic force microscopy

Yuri L Lyubchenko, Luda S. Shlyakhtenko

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.

Original languageEnglish (US)
Pages (from-to)27-42
Number of pages16
JournalMethods in Molecular Biology
StatePublished - 2015


  • AFM
  • Atomic force microscopy
  • Chromatin dynamics
  • Nucleosome dynamics
  • Single-molecule techniques
  • Time-lapse AFM

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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