Chromatin imaging with time-lapse atomic force microscopy

Yuri L. Lyubchenko, Luda S. Shlyakhtenko

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.

Original languageEnglish (US)
Title of host publicationChromatin Protocols
Subtitle of host publicationThird Edition
PublisherSpringer New York
Pages27-42
Number of pages16
ISBN (Electronic)9781493924745
ISBN (Print)9781493924738
DOIs
StatePublished - Mar 31 2015

Keywords

  • AFM
  • Atomic force microscopy
  • Chromatin dynamics
  • Nucleosome dynamics
  • Single-molecule techniques
  • Time-lapse AFM

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Lyubchenko, Y. L., & Shlyakhtenko, L. S. (2015). Chromatin imaging with time-lapse atomic force microscopy. In Chromatin Protocols: Third Edition (pp. 27-42). Springer New York. https://doi.org/10.1007/978-1-4939-2474-5_3