Chromatin imaging with time-lapse atomic force microscopy

Yuri L. Lyubchenko; Luda S. Shlyakhtenko

doi:10.1007/978-1-4939-2474-5_3

Chromatin imaging with time-lapse atomic force microscopy

Yuri L. Lyubchenko, Luda S. Shlyakhtenko

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.

Original language	English (US)
Title of host publication	Chromatin Protocols
Subtitle of host publication	Third Edition
Publisher	Springer New York
Pages	27-42
Number of pages	16
ISBN (Electronic)	9781493924745
ISBN (Print)	9781493924738
DOIs	https://doi.org/10.1007/978-1-4939-2474-5_3
State	Published - Mar 31 2015

Keywords

AFM
Atomic force microscopy
Chromatin dynamics
Nucleosome dynamics
Single-molecule techniques
Time-lapse AFM

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

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10.1007/978-1-4939-2474-5_3

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title = "Chromatin imaging with time-lapse atomic force microscopy",

abstract = "Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.",

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AU - Shlyakhtenko, Luda S.

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PY - 2015/3/31

Y1 - 2015/3/31

N2 - Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.

AB - Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.

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KW - Single-molecule techniques

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Chromatin imaging with time-lapse atomic force microscopy

Abstract

Keywords

ASJC Scopus subject areas

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