Abstract
Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the highspeed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.
Original language | English (US) |
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Title of host publication | Chromatin Protocols |
Subtitle of host publication | Third Edition |
Publisher | Springer New York |
Pages | 27-42 |
Number of pages | 16 |
ISBN (Electronic) | 9781493924745 |
ISBN (Print) | 9781493924738 |
DOIs | |
State | Published - Mar 31 2015 |
Keywords
- AFM
- Atomic force microscopy
- Chromatin dynamics
- Nucleosome dynamics
- Single-molecule techniques
- Time-lapse AFM
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Medicine