Chromatin isolation by RNA purification (ChIRP) and its applications

Changhai Tian; Guoku Hu

doi:10.1016/B978-0-12-819414-0.00025-2

Chromatin isolation by RNA purification (ChIRP) and its applications

Changhai Tian, Guoku Hu

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Chromatin isolation by RNA purification (ChIRP) is a valuable method to map the genomic binding profile of chromatin-associated RNAs, such as long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). ChIRP was initially designed by Chu and colleagues to investigate RNA bound DNA using affinity-probes to a target RNA, and its application has been recently extended to also identify RNAs and proteins that interact with the target RNA. Since its original development in 2011, ChIRP has been increasingly used in various fields of biological research which has, in turn, resulted in an enhanced understanding of the dynamics of RNA-chromatin interactions and their roles in health and diseases. This chapter describes the methodology and applications of ChIRP.

Original language	English (US)
Title of host publication	Epigenetics Methods
Publisher	Elsevier
Pages	507-521
Number of pages	15
ISBN (Electronic)	9780128194140
DOIs	https://doi.org/10.1016/B978-0-12-819414-0.00025-2
State	Published - Jan 1 2020

Keywords

ChIRP
Chromatin
Genome-wide binding
LncRNA
RNA-chromatin interaction

ASJC Scopus subject areas

General Medicine
General Biochemistry, Genetics and Molecular Biology

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10.1016/B978-0-12-819414-0.00025-2

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abstract = "Chromatin isolation by RNA purification (ChIRP) is a valuable method to map the genomic binding profile of chromatin-associated RNAs, such as long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). ChIRP was initially designed by Chu and colleagues to investigate RNA bound DNA using affinity-probes to a target RNA, and its application has been recently extended to also identify RNAs and proteins that interact with the target RNA. Since its original development in 2011, ChIRP has been increasingly used in various fields of biological research which has, in turn, resulted in an enhanced understanding of the dynamics of RNA-chromatin interactions and their roles in health and diseases. This chapter describes the methodology and applications of ChIRP.",

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AB - Chromatin isolation by RNA purification (ChIRP) is a valuable method to map the genomic binding profile of chromatin-associated RNAs, such as long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). ChIRP was initially designed by Chu and colleagues to investigate RNA bound DNA using affinity-probes to a target RNA, and its application has been recently extended to also identify RNAs and proteins that interact with the target RNA. Since its original development in 2011, ChIRP has been increasingly used in various fields of biological research which has, in turn, resulted in an enhanced understanding of the dynamics of RNA-chromatin interactions and their roles in health and diseases. This chapter describes the methodology and applications of ChIRP.

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KW - Genome-wide binding

KW - LncRNA

KW - RNA-chromatin interaction

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Chromatin isolation by RNA purification (ChIRP) and its applications

Abstract

Keywords

ASJC Scopus subject areas

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