TY - JOUR
T1 - Chromatographic studies of protein-based chiral separations
AU - Bi, Cong
AU - Zheng, Xiwei
AU - Azaria, Shiden
AU - Beeram, Sandya
AU - Li, Zhao
AU - Hage, David S.
N1 - Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland. T.
PY - 2016/9
Y1 - 2016/9
N2 - The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.
AB - The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.
KW - Chiral high-performance liquid chromatography (HPLC)
KW - Chiral recognition
KW - Chromatographic studies of drug-protein interactions
KW - Frontal analysis
KW - Protein-based chiral stationary phases
KW - Zonal elution
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U2 - 10.3390/separations3030027
DO - 10.3390/separations3030027
M3 - Review article
C2 - 28344977
AN - SCOPUS:85039841865
SN - 2297-8739
VL - 3
JO - Separations
JF - Separations
IS - 3
M1 - 27
ER -